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KMID : 1161420130160030259
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Journal of Medicinal Food
2013 Volume.16 No. 3 p.259 ~ p.262
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Pu-Erh Tea Hot-Water Extract Activates Akt and Induces Insulin-Independent Glucose Transport in Rat Skeletal Muscle
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Ma Xiao
Tsuda Satoshi
Yang Xin
Gu Ning
Tanabe Hiroko
Oshima Rieko
Matsushita Tetsuya
Egawa Tatsuro
Dong Ai-Jun
Zhu Bei-Wei
Hayashi Tatsuya
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Abstract
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Skeletal muscle is a major organ that is important for whole-body glucose metabolism. We found that when isolated rat epitrochlearis muscle was incubated with a Pu-erh tea hot-water extract (PTE) for 30?min, the rate of 3-O-methyl-D-glucose (3MG) transport increased in the absence of insulin. This activation was associated with an increase in Ser473 phosphorylation of Akt, a signaling intermediary leading to insulin-dependent glucose transport, but not Tyr458 phosphorylation of phosphoinositide 3-kinase p85, an upstream molecule of Akt. PTE-stimulated 3MG transport was also not accompanied by Thr172 phosphorylation of the catalytic α-subunit of 5′-AMP-activated protein kinase (AMPK). Gallic acid, a water-soluble ingredient in Pu-erh tea, stimulated Akt phosphorylation, but not AMPK phosphorylation. These results suggest that Pu-erh tea potentially promotes skeletal muscle glucose transport at least in part by activating Akt.
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KEYWORD
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AMPK, epigallocatechin gallate, glucose metabolism, health functional food, signal transduction
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